Composite

Part:BBa_K3052032:Design

Designed by: Ma WenHe   Group: iGEM19_XJTU-CHINA   (2019-10-14)


Light-fluoresce circuit (GFP) - RXY


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 4619
    Illegal PstI site found at 5168
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 6036
    Illegal NheI site found at 6059
    Illegal PstI site found at 4619
    Illegal PstI site found at 5168
    Illegal NotI site found at 4866
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2298
    Illegal BglII site found at 5391
    Illegal BglII site found at 7596
    Illegal BamHI site found at 5173
    Illegal XhoI site found at 405
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 4619
    Illegal PstI site found at 5168
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 4619
    Illegal PstI site found at 5168
    Illegal NgoMIV site found at 7149
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The expression level of the two phases of this switch may be different.



Source

This part was acquired from chemical synthesis.

References